Download Ayahuasca Analogues Pangean Entheogens (1st edition) by Jonathan Ott PDF

By Jonathan Ott

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""Ayahuasca Analogs" is attention-grabbing, yet for the main half, every little thing during this publication got here from "Pharmacotheon."... the single considerable distinction is that Ayahuasca Analogs comprises extra ethnic recipes for harmaline-tryptamine interplay. "

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1993). 1 ppm 2,4-D. After this treatment the cultures were transferred into a medium of the same composition from which, however, 2,4-D was omitted to induce the realization phase of embryogenesis. At inspection of these cultures about 3 weeks later it was obvious that only those cultures kept 0* Sucrose Glucose Fructose Maltose e se se se se se se se se (se) (se) se culture conditions: liquid medium, 28°C, continuous light, modified BS-rnedium (Schiifer et al. 1988). S ppm 2,4-D BS-: 18 days in BS-medium without 2,4-D 0*: medium without carbohydrates se: somatic embryogenesis e: somatic embryogenesis was induced in auxin-free medium (later induction) -: no somatic embryos (se): minimal formation of somatic embryos in a sucrose containing medium were able to produce adventitious roots and somatic embryos (Figs 3, 4).

6 4 Fig. 11. Time course of the accumulation of podophyllotoxin-(3-D-glucoside in L. ), "(- (0), dimethyl-(3-(e) and hydroxypropyl-(3-cyclodextrin (0) (from Van Uden et al. 1993). 8,-------------------------- 3% of the added podophyllotoxin was detectable at that time. The glucosylation of podophyllotoxin started quickly. 001 % DW) in the cells. The addition of the highly cytotoxic podophyllotoxin to the growth medium of L. ftavum cell suspensions, probably stimulated the cells to detoxify the lignan.

1980) and Yamada et al. (1981) used mechanically agitated bioreactors for the cultivation of photoautotrophic cell suspensions on the 5 I scale. As far as we know, no experiments exceeding the capacity of these bioreactor systems were performed with this type of cells. Therefore, a 20 I system for the growth of photoautotrophic sus- pension cultures has been established. The bioreactor has been running in a long-term semicontinuous mode, because the use of shake-flask grown cell material for inoculation could have provoked at least transient negative scale-up effects.

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