Download DNA-Based Markers in Plants by Benjamin Burr (auth.), Ronald L. Phillips, Indra K. Vasil PDF

By Benjamin Burr (auth.), Ronald L. Phillips, Indra K. Vasil (eds.)

The double helix structure of DNA was once elucidated in 1953. two decades later, in 1973, the invention of limit enzymes helped to create recombinant DNA mol­ ecules in vitro. the consequences of those robust and novel tools of molecular biol­ ogy, and their power within the genetic manipulation and development of microbes, vegetation and animals, grew to become more and more obvious, and ended in the delivery of recent biotechnology. the 1st transgenic crops during which a bacterial gene were stably built-in have been produced in 1983, and through 1993 transgenic crops have been produced in all significant crop species, together with the cereals and the legumes. those outstanding achievements have ended in the construction of vegetation which are immune to powerful yet environmentally secure herbicides, or to viral pathogens and bug pests. In different cases genes were brought that hold up fruit ripening, or raise starch content material, or reason male sterility. each one of these manipulations are in line with the advent of a unmarried gene - typically of bacterial foundation - that regulates a big monogenic trait, into the crop of selection. some of the engineered plants at the moment are lower than box trials and are anticipated to be commercially produced in the following couple of years.

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The most obvious is that markers polymorphic in one mapping population may not show variation in a second population. The first genetic maps were based on mapping populations optimized for DNA polymorphisms, often including parents from distinct, but cross-compatible species. D. Young for lack of polymorphism. When this happens it will be difficult to relate genetic map location between populations, except by cloning sequences that flank the original marker (a substantial amount of effort) or by testing adjacent DNA markers in hopes that they show more sequence variation.

Simplicity and speed are absolutely essential for processing large numbers of individuals - an obvious necessity when large populations of several hundred, or even thousands, of individuals need to be examined. amounts of starting material are advantageous if larger quantities are hard to obtain, such as seeds, seedlings, or physically small plants like Arabidopsis. DNA used for genetic mapping does not need to be highly purified. As long as an extraction provides DNA in sufficient quantity and quality for restriction enzyme digestion or as a template for PCR, the method is probably satisfactory.

1988; Gebhardt et al. 1991; Tanksley et al. 1993), sorghum maps constructed with maize markers (Hulbert et al. 1990; Pereira et al. 1994), a turnip map constructed with markers from cabbage (McGrath and Quiros, 1991), and a mungbean map constructed with markers from both soybean and common bean (Menancio-Hautea et al. 1993). Not only does a pre-existing map provide a set of previously tested DNA markers, it also gives an indication of linkage groups and marker order. In the case of tomato and potato, only five paracentric inversions involving complete chromosome arms differentiate the two maps (Bonierbale et al.

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